cited2  (R&D Systems)

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Cited2 inhibited hypoxia-induced proliferation and migration of PASMCs via the TGF-β1/Cited2/PPARγ pathway

doi: 10.22038/IJBMS.2023.74455.16178

Figure Lengend Snippet: Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (Cited2) expression was down-regulated in hypoxia-induced pulmonary hypertension (HPH) rat models

Article Snippet: A protein-free quick blocking solution (Boster, AR0041) was used to block the membrane at room temperature for 1 hr, and then primary antibodies against Cited2 (1:500, Affinity), anti-PPARγ (1:2000, Proteintech), and anti-β-actin (1:3000, Affinity) were used to incubate the membranes overnight at 4 ° C. On the next day, HRP-labeled secondary antibody (1:5000, Affinity) was used to incubate the membranes at room temperature for 1 hr, and the chemiluminescence method was used for color development.

Techniques: Expressing

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Cited2 inhibited hypoxia-induced proliferation and migration of PASMCs via the TGF-β1/Cited2/PPARγ pathway

doi: 10.22038/IJBMS.2023.74455.16178

Figure Lengend Snippet: Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (Cited2) expression was down-regulated in pulmonary artery smooth muscle cells (PASMCs) in hypoxia

Article Snippet: A protein-free quick blocking solution (Boster, AR0041) was used to block the membrane at room temperature for 1 hr, and then primary antibodies against Cited2 (1:500, Affinity), anti-PPARγ (1:2000, Proteintech), and anti-β-actin (1:3000, Affinity) were used to incubate the membranes overnight at 4 ° C. On the next day, HRP-labeled secondary antibody (1:5000, Affinity) was used to incubate the membranes at room temperature for 1 hr, and the chemiluminescence method was used for color development.

Techniques: Expressing

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Cited2 inhibited hypoxia-induced proliferation and migration of PASMCs via the TGF-β1/Cited2/PPARγ pathway

doi: 10.22038/IJBMS.2023.74455.16178

Figure Lengend Snippet: Effects of Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (Cited2) overexpression on pulmonary artery smooth muscle cells (PASMCs)

Article Snippet: A protein-free quick blocking solution (Boster, AR0041) was used to block the membrane at room temperature for 1 hr, and then primary antibodies against Cited2 (1:500, Affinity), anti-PPARγ (1:2000, Proteintech), and anti-β-actin (1:3000, Affinity) were used to incubate the membranes overnight at 4 ° C. On the next day, HRP-labeled secondary antibody (1:5000, Affinity) was used to incubate the membranes at room temperature for 1 hr, and the chemiluminescence method was used for color development.

Techniques: Over Expression

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Cited2 inhibited hypoxia-induced proliferation and migration of PASMCs via the TGF-β1/Cited2/PPARγ pathway

doi: 10.22038/IJBMS.2023.74455.16178

Figure Lengend Snippet: Effects of Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (Cited2) knockdown on pulmonary artery smooth muscle cells (PASMCs)

Article Snippet: A protein-free quick blocking solution (Boster, AR0041) was used to block the membrane at room temperature for 1 hr, and then primary antibodies against Cited2 (1:500, Affinity), anti-PPARγ (1:2000, Proteintech), and anti-β-actin (1:3000, Affinity) were used to incubate the membranes overnight at 4 ° C. On the next day, HRP-labeled secondary antibody (1:5000, Affinity) was used to incubate the membranes at room temperature for 1 hr, and the chemiluminescence method was used for color development.

Techniques: Knockdown

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Cited2 inhibited hypoxia-induced proliferation and migration of PASMCs via the TGF-β1/Cited2/PPARγ pathway

doi: 10.22038/IJBMS.2023.74455.16178

Figure Lengend Snippet: Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (Cited2) reversed Transforming growth factor-beta1 (TGF-β1)-induced proliferation and migration of pulmonary artery smooth muscle cells (PASMCs)

Article Snippet: A protein-free quick blocking solution (Boster, AR0041) was used to block the membrane at room temperature for 1 hr, and then primary antibodies against Cited2 (1:500, Affinity), anti-PPARγ (1:2000, Proteintech), and anti-β-actin (1:3000, Affinity) were used to incubate the membranes overnight at 4 ° C. On the next day, HRP-labeled secondary antibody (1:5000, Affinity) was used to incubate the membranes at room temperature for 1 hr, and the chemiluminescence method was used for color development.

Techniques: Migration

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Cited2 inhibited hypoxia-induced proliferation and migration of PASMCs via the TGF-β1/Cited2/PPARγ pathway

doi: 10.22038/IJBMS.2023.74455.16178

Figure Lengend Snippet: Peroxisome proliferatoractivated receptor gamma (PPARγ) cooperates with Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (Cited2) to inhibit the proliferation and migration of pulmonary artery smooth muscle cells (PASMCs)

Article Snippet: A protein-free quick blocking solution (Boster, AR0041) was used to block the membrane at room temperature for 1 hr, and then primary antibodies against Cited2 (1:500, Affinity), anti-PPARγ (1:2000, Proteintech), and anti-β-actin (1:3000, Affinity) were used to incubate the membranes overnight at 4 ° C. On the next day, HRP-labeled secondary antibody (1:5000, Affinity) was used to incubate the membranes at room temperature for 1 hr, and the chemiluminescence method was used for color development.

Techniques: Migration

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Cited2 inhibited hypoxia-induced proliferation and migration of PASMCs via the TGF-β1/Cited2/PPARγ pathway

doi: 10.22038/IJBMS.2023.74455.16178

Figure Lengend Snippet: Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (Cited2) expression was down-regulated in hypoxia-induced pulmonary hypertension (HPH) rat models

Article Snippet: Afterward, goat serum was used to block the cells for 1 hr, followed by incubation with Cited2 antibody overnight at 4 ° C. On the following day, CoraLite594-labeled goat anti-mouse secondary antibody (1:100, Proteintech) was used to incubate the cells for 1 hr at room temperature in the dark.

Techniques: Expressing

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Cited2 inhibited hypoxia-induced proliferation and migration of PASMCs via the TGF-β1/Cited2/PPARγ pathway

doi: 10.22038/IJBMS.2023.74455.16178

Figure Lengend Snippet: Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (Cited2) expression was down-regulated in pulmonary artery smooth muscle cells (PASMCs) in hypoxia

Article Snippet: Afterward, goat serum was used to block the cells for 1 hr, followed by incubation with Cited2 antibody overnight at 4 ° C. On the following day, CoraLite594-labeled goat anti-mouse secondary antibody (1:100, Proteintech) was used to incubate the cells for 1 hr at room temperature in the dark.

Techniques: Expressing

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Cited2 inhibited hypoxia-induced proliferation and migration of PASMCs via the TGF-β1/Cited2/PPARγ pathway

doi: 10.22038/IJBMS.2023.74455.16178

Figure Lengend Snippet: Effects of Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (Cited2) overexpression on pulmonary artery smooth muscle cells (PASMCs)

Article Snippet: Afterward, goat serum was used to block the cells for 1 hr, followed by incubation with Cited2 antibody overnight at 4 ° C. On the following day, CoraLite594-labeled goat anti-mouse secondary antibody (1:100, Proteintech) was used to incubate the cells for 1 hr at room temperature in the dark.

Techniques: Over Expression

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Cited2 inhibited hypoxia-induced proliferation and migration of PASMCs via the TGF-β1/Cited2/PPARγ pathway

doi: 10.22038/IJBMS.2023.74455.16178

Figure Lengend Snippet: Effects of Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (Cited2) knockdown on pulmonary artery smooth muscle cells (PASMCs)

Article Snippet: Afterward, goat serum was used to block the cells for 1 hr, followed by incubation with Cited2 antibody overnight at 4 ° C. On the following day, CoraLite594-labeled goat anti-mouse secondary antibody (1:100, Proteintech) was used to incubate the cells for 1 hr at room temperature in the dark.

Techniques: Knockdown

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Cited2 inhibited hypoxia-induced proliferation and migration of PASMCs via the TGF-β1/Cited2/PPARγ pathway

doi: 10.22038/IJBMS.2023.74455.16178

Figure Lengend Snippet: Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (Cited2) reversed Transforming growth factor-beta1 (TGF-β1)-induced proliferation and migration of pulmonary artery smooth muscle cells (PASMCs)

Article Snippet: Afterward, goat serum was used to block the cells for 1 hr, followed by incubation with Cited2 antibody overnight at 4 ° C. On the following day, CoraLite594-labeled goat anti-mouse secondary antibody (1:100, Proteintech) was used to incubate the cells for 1 hr at room temperature in the dark.

Techniques: Migration

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Cited2 inhibited hypoxia-induced proliferation and migration of PASMCs via the TGF-β1/Cited2/PPARγ pathway

doi: 10.22038/IJBMS.2023.74455.16178

Figure Lengend Snippet: Peroxisome proliferatoractivated receptor gamma (PPARγ) cooperates with Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 2 (Cited2) to inhibit the proliferation and migration of pulmonary artery smooth muscle cells (PASMCs)

Article Snippet: Afterward, goat serum was used to block the cells for 1 hr, followed by incubation with Cited2 antibody overnight at 4 ° C. On the following day, CoraLite594-labeled goat anti-mouse secondary antibody (1:100, Proteintech) was used to incubate the cells for 1 hr at room temperature in the dark.

Techniques: Migration

Journal: Frontiers in Neurology

Article Title: VEGF to CITED2 ratio predicts the collateral circulation of acute ischemic stroke

doi: 10.3389/fneur.2022.1000992

Figure Lengend Snippet: Schedule of enrollment and assessments. DWI-ASPECTS, diffusion weight image–Alberta Stroke Program Early CT Score; CITED2, CBP/P300-interacting transactivator with Glu/Asp-rich C-terminal domain 2; VEGF, vascular endothelial growth factor; WB, Western blot; NIHSS, National Institute of Health Stroke Scale; MBI, Modified Barthel Index; mRS, Modified Rankin Scale.

Article Snippet: The membrane was probed with diluted primary antibodies CITED2 (Abclonal, 1:1,000), VEGF (Bioss, 1:1,000), and β-actin (Bioworld, 1:10,000) overnight at 4°C.

Techniques: Diffusion-based Assay, Western Blot, Modification

Journal: Frontiers in Neurology

Article Title: VEGF to CITED2 ratio predicts the collateral circulation of acute ischemic stroke

doi: 10.3389/fneur.2022.1000992

Figure Lengend Snippet: Patient selection. DWI-ASPECTS, diffusion weight image–Alberta Stroke Program Early CT Score; CITED2, CBP/P300-interacting transactivator with Glu/Asp-rich C-terminal domain 2; VEGF, vascular endothelial growth factor; WB, Western blot.

Article Snippet: The membrane was probed with diluted primary antibodies CITED2 (Abclonal, 1:1,000), VEGF (Bioss, 1:1,000), and β-actin (Bioworld, 1:10,000) overnight at 4°C.

Techniques: Selection, Diffusion-based Assay, Western Blot

Journal: Frontiers in Neurology

Article Title: VEGF to CITED2 ratio predicts the collateral circulation of acute ischemic stroke

doi: 10.3389/fneur.2022.1000992

Figure Lengend Snippet: Protein expression of CITED2 and VEGF.

Article Snippet: The membrane was probed with diluted primary antibodies CITED2 (Abclonal, 1:1,000), VEGF (Bioss, 1:1,000), and β-actin (Bioworld, 1:10,000) overnight at 4°C.

Techniques: Expressing

Journal: Frontiers in Neurology

Article Title: VEGF to CITED2 ratio predicts the collateral circulation of acute ischemic stroke

doi: 10.3389/fneur.2022.1000992

Figure Lengend Snippet: Clinical data of the included patients.

Article Snippet: The membrane was probed with diluted primary antibodies CITED2 (Abclonal, 1:1,000), VEGF (Bioss, 1:1,000), and β-actin (Bioworld, 1:10,000) overnight at 4°C.

Techniques: Significance Assay

Journal: Frontiers in Neurology

Article Title: VEGF to CITED2 ratio predicts the collateral circulation of acute ischemic stroke

doi: 10.3389/fneur.2022.1000992

Figure Lengend Snippet: Univariate logistic regression analysis of collateral circulation-related factors in AIS.

Article Snippet: The membrane was probed with diluted primary antibodies CITED2 (Abclonal, 1:1,000), VEGF (Bioss, 1:1,000), and β-actin (Bioworld, 1:10,000) overnight at 4°C.

Techniques:

Journal: Frontiers in Neurology

Article Title: VEGF to CITED2 ratio predicts the collateral circulation of acute ischemic stroke

doi: 10.3389/fneur.2022.1000992

Figure Lengend Snippet: Multivariate logistic regression analysis of collateral circulation-related factors in AIS.

Article Snippet: The membrane was probed with diluted primary antibodies CITED2 (Abclonal, 1:1,000), VEGF (Bioss, 1:1,000), and β-actin (Bioworld, 1:10,000) overnight at 4°C.

Techniques:

Journal: Frontiers in Neurology

Article Title: VEGF to CITED2 ratio predicts the collateral circulation of acute ischemic stroke

doi: 10.3389/fneur.2022.1000992

Figure Lengend Snippet: ROC curve analysis of the VEGF/CITED2 in the collateral circulation of AIS.

Article Snippet: The membrane was probed with diluted primary antibodies CITED2 (Abclonal, 1:1,000), VEGF (Bioss, 1:1,000), and β-actin (Bioworld, 1:10,000) overnight at 4°C.

Techniques:

Journal: Frontiers in Neurology

Article Title: VEGF to CITED2 ratio predicts the collateral circulation of acute ischemic stroke

doi: 10.3389/fneur.2022.1000992

Figure Lengend Snippet: ROC analysis of the poor collateral circulation in AIS.

Article Snippet: The membrane was probed with diluted primary antibodies CITED2 (Abclonal, 1:1,000), VEGF (Bioss, 1:1,000), and β-actin (Bioworld, 1:10,000) overnight at 4°C.

Techniques: